Shadow effect
The shadow effect is a phenomenon seen in genetic studies that use noninvasive genetic data collection methods. It occurs when there are not enough loci and/or loci that have low variance of alleles within the population. As a result, researchers can capture two separate individuals and mistakenly label them as the same individual. This can create a negative bias in the data and portray a population as smaller and less genetically diverse than it is. This is most commonly seen in collection methods that rely on environmental DNA (eDNA) which is collected directly from the environment. The accuracy of non-invasive collection data can be increased by increasing the amount of loci being examined during the study.
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